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M94A2654.TXT
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1994-10-25
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Document 2654
DOCN M94A2654
TI Efficacy of HIV antigen acid dissociation in pediatric AIDS.
DT 9412
AU Canizal A; Zarwanitzer S; Benetucci J; Laube G; Rubinstein E; Sosa CE;
Laboratorio de Retrovirus, FUNDAI y Pediatria, Buenos Aires.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):255 (abstract no. PB0448). Unique
Identifier : AIDSLINE ICA10/94369921
AB OBJETIVES: To asses the efficacy of acid pretreatment in increasing
sensitivity of p24 Antigen detection. METHODS: Forty four sera from
children born to seropositive mothers were investigated with or without
acid pretreatment and compared to HIV co-culture as a standard. We used
CIH (pH 2-3) to disrupt immunocomplexes. Samples were incubated for one
hour followed by neutralization with NaOH. EIA was performed and
positive specimens were confirmed by a commercial kit (Organon,
Teknika). RESULTS: Age of children ranged from 1 to 24 months (mean
7.2). Clinical status: P0 n = 27; P1 n = 1; P2 n = 16. Twelve children
from whom pretreated antigen was detected were asymptomatic (P0 status)
and below six months of age. Noteworthy, pretreated antigen was detected
in four samples which were negative co-culture. TABULAR DATA, SEE
ABSTRACT VOLUME. CONCLUSIONS: Acid pretreatment increased the
sensitivity of the assay from 40.9% to 72.7%. This procedure showed to
be very valuable for early diagnosis of HIV infected children, specially
for developing countries.
DE Acquired Immunodeficiency Syndrome/*DIAGNOSIS/IMMUNOLOGY/ TRANSMISSION
Antigen-Antibody Complex/*IMMUNOLOGY *AIDS Serodiagnosis Female
Follow-Up Studies Human HIV Core Protein p24/*BLOOD Immunoenzyme
Techniques Infant Infant, Newborn Predictive Value of Tests
Pregnancy MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).